Development of a Competitive Lateral Flow Immunoassay for Progesterone in Dairy Cows’ Milk

Pretreatment-free lateral flow enzyme immunoassay for progesterone detection in whole cows' milk.

New rapid method of lateral flow enzyme immunoassay (LFEIA) for progesterone detection in whole cows' milk was developed. The test system utilized horseradish peroxidase as a label along with the substrate solution containing 3,3',5,5'-tetramethylbenzidine and dextran sulfate to obtain an insoluble blue colored product of the enzyme reaction on a surface of analytical membrane (test and control...

Development of a competitive lateral flow immunoassay for progesterone: influence of coating conjugates and buffer components.

Several aspects of the development of competitive lateral flow immunoassays (LFIAs) are described. The quantitation of progesterone is taken as an example. The LFIA format consisted of a nitrocellulose membrane spotted with various progesterone conjugates as the test line. A mixture of primary antibody and secondary antibody adsorbed to colloidal carbon was used for signal generation. A digital...

Enzyme-immunoassay for determination of progesterone in milk from cows.

An enzyme-immunoassay using horseradish peroxidase as label is described. The assay has a sensitivity of 0.1-20 pmol, i.e. 0.03-0.63 ng and intra- and inter-assay coefficients of variation of less than 10% when used for the determination of progesterone in samples of milk from cows. Comparison with a radio-immunoassay based on tritiated tracer showed excellent correlation (r = 0.98) for milk sa...

Measuring milk flow of dairy cows.

Milk flow measurements on 124 cows were analyzed to deternfine how they are affected by vacuum level, pulsation rate, pulsation ratio of the milking maehine, and the breed, age, stage of laetation, and milk yield of the cow. Average rate of flow prior to machine stripping, yield during the first minute, and yield during the first 2 rain of milking were highly correlated with maxinmm rate of mil...

Milk progesterone measures to improve genomic selection for fertility in dairy cows